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lteto 1 promoter  (Addgene inc)


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    Structured Review

    Addgene inc lteto 1 promoter
    Lteto 1 Promoter, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 135 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pdcas9-bacteria+plasmid/pdCas9-bacteria+(Plasmid+%2344249)/pmc11613459-266-29-37
    Average 94 stars, based on 135 article reviews
    lteto 1 promoter - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Virus:

    Article Title: CRISPR-based peptide library display and programmable microarray self-assembly for rapid quantitative protein binding assays.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-6His, rabbit Cell Signaling D3I1O; RRID: AB_2744546 Anti-HA, rabbit Cell Signaling C29F4; RRID: AB_1549585 Anti-myc, rabbit Abcam ab9106; RRID: AB_307014 Anti-FLAG M2, mouse Millipore Sigma F1804; RRID: AB_262044 Anti-FLAG, rabbit Abcam ab205606 Goat anti-mouse IgG (H&L)-Alexa 647 Abcam ab150115; RRID: AB_2687948 Goat anti-rabbit IgG (H&L)-Alexa 488 Abcam ab150077; RRID: AB_2630356 Donkey anti-rabbit IgG (H&L)-Alexa 647 Abcam ab150075; RRID: AB_2752244 Goat anti-human IgG (H&L)-Alexa 647 ThermoFisher A-21445; RRID: AB_2535862 Bacterial and virus strains Electromax DH10B E. coli ThermoFisher 18290015 BL21 (DE3) electrocompetent E. coli Sigma-Aldrich CMC0016 T7 Express lysY Competent E. coli NEB C3010I Deposited data Raw microarray image files This paper https://doi.org/10.17632/rz8ty3g3yf.1 Microarray data processing scripts (original code) This paper https://doi.org/10.17632/3kg89nrmwk.1 Link: https://github.com/kwbarber/ PICASSO Oligonucleotides SurePrint Oligonucleotide Libraries listed in Table S2 This paper (synthesized by Agilent) G7220A Oligonucleotide microarrays as PICASSO templates This paper (synthesized by CustomArray/ Genscript) N/A Primer to doublestrand dsDNAmicroarrays: 50-AC+G+G+GT+GAGGTCGGG-30, where + denotes LNA bases This paper (synthesized by IDT) N/A Recombinant DNA pdCas9-bacteria plasmid Qi et al., 2013 Addgene #44249 pgRNA-bacteria Qi et al., 2013 Addgene #44251 dCas9-fusion+sgRNA library expression vector (see Data S1) This paper Addgene #171798 dCas9-fusion+sgRNA expression scaffold (see Data S1) This paper Addgene #171799 Software and algorithms GenePix Pro 7 Molecular Devices N/A Other GenePix 4300A microarray scanner Molecular Devices GENEPIX 4300 Detailed Protocol This paper Methods S1

    Microarray:

    Article Title: CRISPR-based peptide library display and programmable microarray self-assembly for rapid quantitative protein binding assays.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-6His, rabbit Cell Signaling D3I1O; RRID: AB_2744546 Anti-HA, rabbit Cell Signaling C29F4; RRID: AB_1549585 Anti-myc, rabbit Abcam ab9106; RRID: AB_307014 Anti-FLAG M2, mouse Millipore Sigma F1804; RRID: AB_262044 Anti-FLAG, rabbit Abcam ab205606 Goat anti-mouse IgG (H&L)-Alexa 647 Abcam ab150115; RRID: AB_2687948 Goat anti-rabbit IgG (H&L)-Alexa 488 Abcam ab150077; RRID: AB_2630356 Donkey anti-rabbit IgG (H&L)-Alexa 647 Abcam ab150075; RRID: AB_2752244 Goat anti-human IgG (H&L)-Alexa 647 ThermoFisher A-21445; RRID: AB_2535862 Bacterial and virus strains Electromax DH10B E. coli ThermoFisher 18290015 BL21 (DE3) electrocompetent E. coli Sigma-Aldrich CMC0016 T7 Express lysY Competent E. coli NEB C3010I Deposited data Raw microarray image files This paper https://doi.org/10.17632/rz8ty3g3yf.1 Microarray data processing scripts (original code) This paper https://doi.org/10.17632/3kg89nrmwk.1 Link: https://github.com/kwbarber/ PICASSO Oligonucleotides SurePrint Oligonucleotide Libraries listed in Table S2 This paper (synthesized by Agilent) G7220A Oligonucleotide microarrays as PICASSO templates This paper (synthesized by CustomArray/ Genscript) N/A Primer to doublestrand dsDNAmicroarrays: 50-AC+G+G+GT+GAGGTCGGG-30, where + denotes LNA bases This paper (synthesized by IDT) N/A Recombinant DNA pdCas9-bacteria plasmid Qi et al., 2013 Addgene #44249 pgRNA-bacteria Qi et al., 2013 Addgene #44251 dCas9-fusion+sgRNA library expression vector (see Data S1) This paper Addgene #171798 dCas9-fusion+sgRNA expression scaffold (see Data S1) This paper Addgene #171799 Software and algorithms GenePix Pro 7 Molecular Devices N/A Other GenePix 4300A microarray scanner Molecular Devices GENEPIX 4300 Detailed Protocol This paper Methods S1

    Synthesized:

    Article Title: CRISPR-based peptide library display and programmable microarray self-assembly for rapid quantitative protein binding assays.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-6His, rabbit Cell Signaling D3I1O; RRID: AB_2744546 Anti-HA, rabbit Cell Signaling C29F4; RRID: AB_1549585 Anti-myc, rabbit Abcam ab9106; RRID: AB_307014 Anti-FLAG M2, mouse Millipore Sigma F1804; RRID: AB_262044 Anti-FLAG, rabbit Abcam ab205606 Goat anti-mouse IgG (H&L)-Alexa 647 Abcam ab150115; RRID: AB_2687948 Goat anti-rabbit IgG (H&L)-Alexa 488 Abcam ab150077; RRID: AB_2630356 Donkey anti-rabbit IgG (H&L)-Alexa 647 Abcam ab150075; RRID: AB_2752244 Goat anti-human IgG (H&L)-Alexa 647 ThermoFisher A-21445; RRID: AB_2535862 Bacterial and virus strains Electromax DH10B E. coli ThermoFisher 18290015 BL21 (DE3) electrocompetent E. coli Sigma-Aldrich CMC0016 T7 Express lysY Competent E. coli NEB C3010I Deposited data Raw microarray image files This paper https://doi.org/10.17632/rz8ty3g3yf.1 Microarray data processing scripts (original code) This paper https://doi.org/10.17632/3kg89nrmwk.1 Link: https://github.com/kwbarber/ PICASSO Oligonucleotides SurePrint Oligonucleotide Libraries listed in Table S2 This paper (synthesized by Agilent) G7220A Oligonucleotide microarrays as PICASSO templates This paper (synthesized by CustomArray/ Genscript) N/A Primer to doublestrand dsDNAmicroarrays: 50-AC+G+G+GT+GAGGTCGGG-30, where + denotes LNA bases This paper (synthesized by IDT) N/A Recombinant DNA pdCas9-bacteria plasmid Qi et al., 2013 Addgene #44249 pgRNA-bacteria Qi et al., 2013 Addgene #44251 dCas9-fusion+sgRNA library expression vector (see Data S1) This paper Addgene #171798 dCas9-fusion+sgRNA expression scaffold (see Data S1) This paper Addgene #171799 Software and algorithms GenePix Pro 7 Molecular Devices N/A Other GenePix 4300A microarray scanner Molecular Devices GENEPIX 4300 Detailed Protocol This paper Methods S1

    Recombinant:

    Article Title: CRISPR-based peptide library display and programmable microarray self-assembly for rapid quantitative protein binding assays.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-6His, rabbit Cell Signaling D3I1O; RRID: AB_2744546 Anti-HA, rabbit Cell Signaling C29F4; RRID: AB_1549585 Anti-myc, rabbit Abcam ab9106; RRID: AB_307014 Anti-FLAG M2, mouse Millipore Sigma F1804; RRID: AB_262044 Anti-FLAG, rabbit Abcam ab205606 Goat anti-mouse IgG (H&L)-Alexa 647 Abcam ab150115; RRID: AB_2687948 Goat anti-rabbit IgG (H&L)-Alexa 488 Abcam ab150077; RRID: AB_2630356 Donkey anti-rabbit IgG (H&L)-Alexa 647 Abcam ab150075; RRID: AB_2752244 Goat anti-human IgG (H&L)-Alexa 647 ThermoFisher A-21445; RRID: AB_2535862 Bacterial and virus strains Electromax DH10B E. coli ThermoFisher 18290015 BL21 (DE3) electrocompetent E. coli Sigma-Aldrich CMC0016 T7 Express lysY Competent E. coli NEB C3010I Deposited data Raw microarray image files This paper https://doi.org/10.17632/rz8ty3g3yf.1 Microarray data processing scripts (original code) This paper https://doi.org/10.17632/3kg89nrmwk.1 Link: https://github.com/kwbarber/ PICASSO Oligonucleotides SurePrint Oligonucleotide Libraries listed in Table S2 This paper (synthesized by Agilent) G7220A Oligonucleotide microarrays as PICASSO templates This paper (synthesized by CustomArray/ Genscript) N/A Primer to doublestrand dsDNAmicroarrays: 50-AC+G+G+GT+GAGGTCGGG-30, where + denotes LNA bases This paper (synthesized by IDT) N/A Recombinant DNA pdCas9-bacteria plasmid Qi et al., 2013 Addgene #44249 pgRNA-bacteria Qi et al., 2013 Addgene #44251 dCas9-fusion+sgRNA library expression vector (see Data S1) This paper Addgene #171798 dCas9-fusion+sgRNA expression scaffold (see Data S1) This paper Addgene #171799 Software and algorithms GenePix Pro 7 Molecular Devices N/A Other GenePix 4300A microarray scanner Molecular Devices GENEPIX 4300 Detailed Protocol This paper Methods S1

    Plasmid Preparation:

    Article Title: CRISPR-based peptide library display and programmable microarray self-assembly for rapid quantitative protein binding assays.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-6His, rabbit Cell Signaling D3I1O; RRID: AB_2744546 Anti-HA, rabbit Cell Signaling C29F4; RRID: AB_1549585 Anti-myc, rabbit Abcam ab9106; RRID: AB_307014 Anti-FLAG M2, mouse Millipore Sigma F1804; RRID: AB_262044 Anti-FLAG, rabbit Abcam ab205606 Goat anti-mouse IgG (H&L)-Alexa 647 Abcam ab150115; RRID: AB_2687948 Goat anti-rabbit IgG (H&L)-Alexa 488 Abcam ab150077; RRID: AB_2630356 Donkey anti-rabbit IgG (H&L)-Alexa 647 Abcam ab150075; RRID: AB_2752244 Goat anti-human IgG (H&L)-Alexa 647 ThermoFisher A-21445; RRID: AB_2535862 Bacterial and virus strains Electromax DH10B E. coli ThermoFisher 18290015 BL21 (DE3) electrocompetent E. coli Sigma-Aldrich CMC0016 T7 Express lysY Competent E. coli NEB C3010I Deposited data Raw microarray image files This paper https://doi.org/10.17632/rz8ty3g3yf.1 Microarray data processing scripts (original code) This paper https://doi.org/10.17632/3kg89nrmwk.1 Link: https://github.com/kwbarber/ PICASSO Oligonucleotides SurePrint Oligonucleotide Libraries listed in Table S2 This paper (synthesized by Agilent) G7220A Oligonucleotide microarrays as PICASSO templates This paper (synthesized by CustomArray/ Genscript) N/A Primer to doublestrand dsDNAmicroarrays: 50-AC+G+G+GT+GAGGTCGGG-30, where + denotes LNA bases This paper (synthesized by IDT) N/A Recombinant DNA pdCas9-bacteria plasmid Qi et al., 2013 Addgene #44249 pgRNA-bacteria Qi et al., 2013 Addgene #44251 dCas9-fusion+sgRNA library expression vector (see Data S1) This paper Addgene #171798 dCas9-fusion+sgRNA expression scaffold (see Data S1) This paper Addgene #171799 Software and algorithms GenePix Pro 7 Molecular Devices N/A Other GenePix 4300A microarray scanner Molecular Devices GENEPIX 4300 Detailed Protocol This paper Methods S1

    Expressing:

    Article Title: CRISPR-based peptide library display and programmable microarray self-assembly for rapid quantitative protein binding assays.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-6His, rabbit Cell Signaling D3I1O; RRID: AB_2744546 Anti-HA, rabbit Cell Signaling C29F4; RRID: AB_1549585 Anti-myc, rabbit Abcam ab9106; RRID: AB_307014 Anti-FLAG M2, mouse Millipore Sigma F1804; RRID: AB_262044 Anti-FLAG, rabbit Abcam ab205606 Goat anti-mouse IgG (H&L)-Alexa 647 Abcam ab150115; RRID: AB_2687948 Goat anti-rabbit IgG (H&L)-Alexa 488 Abcam ab150077; RRID: AB_2630356 Donkey anti-rabbit IgG (H&L)-Alexa 647 Abcam ab150075; RRID: AB_2752244 Goat anti-human IgG (H&L)-Alexa 647 ThermoFisher A-21445; RRID: AB_2535862 Bacterial and virus strains Electromax DH10B E. coli ThermoFisher 18290015 BL21 (DE3) electrocompetent E. coli Sigma-Aldrich CMC0016 T7 Express lysY Competent E. coli NEB C3010I Deposited data Raw microarray image files This paper https://doi.org/10.17632/rz8ty3g3yf.1 Microarray data processing scripts (original code) This paper https://doi.org/10.17632/3kg89nrmwk.1 Link: https://github.com/kwbarber/ PICASSO Oligonucleotides SurePrint Oligonucleotide Libraries listed in Table S2 This paper (synthesized by Agilent) G7220A Oligonucleotide microarrays as PICASSO templates This paper (synthesized by CustomArray/ Genscript) N/A Primer to doublestrand dsDNAmicroarrays: 50-AC+G+G+GT+GAGGTCGGG-30, where + denotes LNA bases This paper (synthesized by IDT) N/A Recombinant DNA pdCas9-bacteria plasmid Qi et al., 2013 Addgene #44249 pgRNA-bacteria Qi et al., 2013 Addgene #44251 dCas9-fusion+sgRNA library expression vector (see Data S1) This paper Addgene #171798 dCas9-fusion+sgRNA expression scaffold (see Data S1) This paper Addgene #171799 Software and algorithms GenePix Pro 7 Molecular Devices N/A Other GenePix 4300A microarray scanner Molecular Devices GENEPIX 4300 Detailed Protocol This paper Methods S1

    Software:

    Article Title: CRISPR-based peptide library display and programmable microarray self-assembly for rapid quantitative protein binding assays.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-6His, rabbit Cell Signaling D3I1O; RRID: AB_2744546 Anti-HA, rabbit Cell Signaling C29F4; RRID: AB_1549585 Anti-myc, rabbit Abcam ab9106; RRID: AB_307014 Anti-FLAG M2, mouse Millipore Sigma F1804; RRID: AB_262044 Anti-FLAG, rabbit Abcam ab205606 Goat anti-mouse IgG (H&L)-Alexa 647 Abcam ab150115; RRID: AB_2687948 Goat anti-rabbit IgG (H&L)-Alexa 488 Abcam ab150077; RRID: AB_2630356 Donkey anti-rabbit IgG (H&L)-Alexa 647 Abcam ab150075; RRID: AB_2752244 Goat anti-human IgG (H&L)-Alexa 647 ThermoFisher A-21445; RRID: AB_2535862 Bacterial and virus strains Electromax DH10B E. coli ThermoFisher 18290015 BL21 (DE3) electrocompetent E. coli Sigma-Aldrich CMC0016 T7 Express lysY Competent E. coli NEB C3010I Deposited data Raw microarray image files This paper https://doi.org/10.17632/rz8ty3g3yf.1 Microarray data processing scripts (original code) This paper https://doi.org/10.17632/3kg89nrmwk.1 Link: https://github.com/kwbarber/ PICASSO Oligonucleotides SurePrint Oligonucleotide Libraries listed in Table S2 This paper (synthesized by Agilent) G7220A Oligonucleotide microarrays as PICASSO templates This paper (synthesized by CustomArray/ Genscript) N/A Primer to doublestrand dsDNAmicroarrays: 50-AC+G+G+GT+GAGGTCGGG-30, where + denotes LNA bases This paper (synthesized by IDT) N/A Recombinant DNA pdCas9-bacteria plasmid Qi et al., 2013 Addgene #44249 pgRNA-bacteria Qi et al., 2013 Addgene #44251 dCas9-fusion+sgRNA library expression vector (see Data S1) This paper Addgene #171798 dCas9-fusion+sgRNA expression scaffold (see Data S1) This paper Addgene #171799 Software and algorithms GenePix Pro 7 Molecular Devices N/A Other GenePix 4300A microarray scanner Molecular Devices GENEPIX 4300 Detailed Protocol This paper Methods S1



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